Quick answer: Trim the clip to zero crossings, import it as PCM (or ADPCM) instead of Vorbis to avoid added padding, and ensure the clip length is an exact loop with no leading/trailing silence.
Every time your ambient loop restarts there's a tiny tick. Looping only sounds seamless when the sample at the end matches the sample at the start; otherwise you hear the discontinuity.
How to fix it
1. Cut on zero crossings
Edit the clip so both ends sit at amplitude zero. A nonzero jump from the last sample to the first is exactly what the click is.
2. Avoid Vorbis padding
Set the import Load Type and Compression Format to PCM for short loops. Vorbis encoding adds priming samples that desync the loop and create a gap.
3. Strip silence
Remove any leading or trailing silence baked into the file; even a few silent samples turn a seamless loop into a gap with a click.
Catching the ones you can't reproduce
The hardest version of this to fix is the one you can't reproduce — it only happens on a player's hardware, OS, driver, or save state, under conditions that simply aren't present on your machine. A report that says “it crashed” or “it froze” gives you nothing to act on, so the bug survives release after release while quietly costing you players.
Automatic error capture closes that gap. Each failure arrives with its full stack trace, the device and OS, the build number, and a breadcrumb trail of what the player did right before it broke, so even a failure you have never seen becomes a specific, reproducible issue. Fold identical failures into one signature ranked by how many players each hits, and your worklist sorts itself worst-first instead of arriving as a stream of vague complaints.
This is where a tool like Bugnet earns its place. Its SDK captures every Unity error automatically with the full stack trace plus device, OS, memory, build, and game-state context, folds duplicates into one grouped issue with an occurrence count, and ties each to the build it first appeared on — so you fix the problem that hurts the most players first and confirm it is gone when its signature disappears from the next release.
Ship the fix, watch the signature disappear from the next build. That's how you know it's really gone.